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Last but not least, 1 μCi of 86Rb was added to each tradition and incubation was ongoing at 37 °C for an extra 10 min. Reactions were being terminated by washing the cells with ice-cold a hundred mM MgCl2 and cell lysates had been geared up for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain had been subtr